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The Patented Mediator Release Test

The Patented Mediator Release Test

The Patented Mediator Release Test

MRT at a Glance

  • Quantifies the inflammatory response to food & food-chemicals
  • Accounts for clinical and subclinical inflammation
  • Accounts for the widest range of inflammatory pathways
  • Capable of measuring both innate and adaptive pathways
Highest Clinical Utility
  • Gives information no other blood test can give
  • Identifies your patients best foods
  • Directly translates into a multi-step eating plan
  • 93.6% split sample reproducibility
  • Awarded 3 US Patents
  • MRT: Canadian Patent No. 2,250,125
  • MRT: U.S. Patent No. 6,114,174
  • Ribbon Method of Cell Analysis: U.S. Patent No. 6,200,815

Why MRT is the Most Complete Blood Test for Sensitivities

Despite all of the clinical and immunologic complexities associated with food sensitivities, the single common component of all diet-induced inflammatory reactions is proinflammatory and proalgesic mediator release from white cells. It’s the release of cytokines, histamine, leukotrienes, prostaglandins, etc., from neutrophils, monocytes, eosinophils and lymphocytes that lead to all the negative clinical effects a food sensitivity sufferer endures. This is true under all of the numerous immunologic circumstances and clinical circumstances associated with food sensitivities. Because of the vast array of potential mediators and reacting cells, measuring volumetric changes in all circulating white cells after antigen challenge is the most logical, direct, comprehensive and functional measure of food sensitivity reactions. It simply makes the most sense.

Research on MRT confirms this. Studies with the University of Miami and research presented at major medical conferences show that MRT is able to distinguish between symptomatic and asymptomatic populations, that MRT correlates with inflammation and symptoms, that diets based on MRT show significant symptom reduction, and that. MRT  has excellent real-world reproducibility.

Conversely, a large body of research has shown that elevated mechanisms in food sensitivity, such as food-specific IgG or immune complexes, do not reliably correlate with inflammation or symptoms.

Mediator release is the key event that leads to every negative effect your patients suffer. What matters clinically is that mediator release, and thus an inflammatory response has occurred – not that a potential mechanism is elevated.

This is the clinical value of MRT . MRT is a functional measurement of diet-induced sensitivity pathways. MRT simplifies a highly complex reaction and translates that into the most useable clinical information you can get – quantifying the inflammatory response to foods and food-chemicals.

Not only does MRT give insight into inflammation provoking foods and food-chemicals, but more importantly MRT identifies your patient’s BEST foods – the foods that form the basis of their LEAP Eating Plan.

Simply put, MRT gives you information you can’t get any other way, and that information directly translates into targeted therapy that matters.

MRT is the foundation of fully addressing food sensitivities and achieving the maximum outcomes in the shortest period of time. This is our goal.

The Patented Mediator Release Test

Use of the LEAP Mediator Release Test to Identify Non-IgE Mediated Immunologic Food Reactions that Trigger Diarrhea Predominant IBS Symptoms Results in Marked Improvement of Symptoms Through Use of an Elimination Diet

American College of Gastroenterology, Annual Scientific & Educational Meeting, Orlando, Florida, November 2004, Fred H. Williams, MD, Department of Gastroenterology, St. John’s Mercy Medical Center, St. Louis, Missouri, United States


Diarrhoea predominant IBS (D-IBS) is a common condition that is often refractory to standard therapy. Though some treatments may improve certain symptoms, there is no treatment that has been shown to result in the improvement of global D-IBS symptoms. The Lifestyle Eating and Performance Mediator Release Test (LEAP MRT) is an in vitro test that detects non-IgE mediated food reactions that can trigger D-IBS symptoms. We report on our early experience with this dietary modification program.


Ten patients who met Rome II criteria for D-IBS are reported in this study. These patients presented to our community-based gastroenterology practice and were evaluated by a gastroenterologist. Evaluation for other causes of their symptoms was based on the patient’s previous evaluation and the discretion of the gastroenterologist. Typically, if not already employed in the past, a trial of standard therapy such as fibre and anti-spasmodic agents was attempted. If the patient didn’t improve, they were then offered LEAP MRT testing. Using an in vitro assay, the patient’s blood was tested for non-IgE mediated reactivity to150 foods and food additives. A specific elimination diet that omitted the reactive foods was then designed for the patient. A Symptom Survey was employed to follow the patients for improvement in D-IBS as well as systemic symptoms. The survey graded multiple GI and systemic symptoms on a scale of 0-4 with increasing severity represented by a higher number. The maximum points possible for the entire survey was 236 and for the GI portion was 36.


Prior to beginning the LEAP MRT based elimination diet, the average score for the entire survey was 56.9 and for the GI portion was 19.1. After at least one month on the diet, the average scores had decreased to 26.3 and 6.3 respectively. Patients generally reported a marked improvement in their D-IBS symptoms, decreased systemic symptoms, and an overall increase in their feeling of well-being.


The LEAP MRT identifies non-IgE mediated immunologic food reactions that trigger D-IBS symptoms. Elimination of these foods from the diet results in a marked improvement in D-IBS and other systemic symptoms.

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